Gmina Kołaczkowo is a rural gmina (administrative district) in Września County, Greater Poland Voivodeship, in west-central Poland. Its seat is the village of Kołaczkowo, which lies approximately 14 kilometres (9 mi) south of Września and 52 km (32 mi) south-east of the regional capital Poznań.
The gmina covers an area of 115.95 square kilometres (44.8 sq mi), and as of 2006 its total population is 6,097.
Gmina Kołaczkowo contains the villages and settlements of Bieganowo, Borzykowo, Budziłowo, Cieśle Małe, Cieśle Wielkie, Gałęzewice, Gorazdowo, Grabowo Królewskie, Kołaczkowo, Krzywa Góra, Łagiewki, Sokolniki, Spławie, Szamarzewo, Wszembórz, Zieliniec and Żydowo
Gmina Kołaczkowo is bordered by the gminas of Lądek, Miłosław, Pyzdry, Strzałkowo, Września and Żerków.