Mysletín is a village and municipality (obec) in Pelhřimov District in the Vysočina Region of the Czech Republic.
The municipality covers an area of 4.14 square kilometres (1.60 sq mi), and has a population of 101 (as at 2 October 2006).
Mysletín lies approximately 13 kilometres (8 mi) north-east of Pelhřimov, 18 km (11 mi) north-west of Jihlava, and 97 km (60 mi) south-east of Prague.